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Addgene inc balazsi g
Balazsi G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids/pDN-D2irTN2AG5kwh+(Plasmid+%23128253)/pm42926929-191-9-11
Average 91 stars, based on 4 article reviews
balazsi g - by Bioz Stars, 2026-11
91/100 stars

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Article Title: Disruption of iron metabolism resulting from Dmt1/Slc11a2 deficiency compromises Notch protein degradation and transcriptional activation.
Article Snippet: The following plasmids were ordered from Addgene (Watertown, MA, USA): pEGFP-p97 (no.: 85670), Lamp1-RFP (no.: 1817), Lamp1-YFP (no.: 1816), and mCherry-Gal3 (no.: 85662).

Bioprocessing:

Article Title: Differential plasticity of excitatory and inhibitory reticulospinal fibers after spinal cord injury: Implication for recovery
Article Snippet: .. The following plasmids were purchased from Addgene (Watertown, MA, USA): AAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362), pAAV-hSyn-DIO-mCherry (#50459). ..

Stable Transfection:

Article Title: A Rab1 interactome illuminates a dual role in autophagy and membrane trafficking
Article Snippet: .. Cells stably expressing the pSu9-Halo-mGFP mitophagy reporter, in addition to the indicated OPTN constructs, were further infected using lentiviral particles carrying YFP-Parkin–expressing plasmids (Addgene_23955) to ensure adequate mitophagy responses in HeLa cells. .. 24 h later these cells were then incubated with DMEM containing 10% FBS and 100 nm TMR HaloTag ligand (Promega) for 20 min at 37°C.

Expressing:

Article Title: A Rab1 interactome illuminates a dual role in autophagy and membrane trafficking
Article Snippet: .. Cells stably expressing the pSu9-Halo-mGFP mitophagy reporter, in addition to the indicated OPTN constructs, were further infected using lentiviral particles carrying YFP-Parkin–expressing plasmids (Addgene_23955) to ensure adequate mitophagy responses in HeLa cells. .. 24 h later these cells were then incubated with DMEM containing 10% FBS and 100 nm TMR HaloTag ligand (Promega) for 20 min at 37°C.

Construct:

Article Title: A Rab1 interactome illuminates a dual role in autophagy and membrane trafficking
Article Snippet: .. Cells stably expressing the pSu9-Halo-mGFP mitophagy reporter, in addition to the indicated OPTN constructs, were further infected using lentiviral particles carrying YFP-Parkin–expressing plasmids (Addgene_23955) to ensure adequate mitophagy responses in HeLa cells. .. 24 h later these cells were then incubated with DMEM containing 10% FBS and 100 nm TMR HaloTag ligand (Promega) for 20 min at 37°C.

Infection:

Article Title: A Rab1 interactome illuminates a dual role in autophagy and membrane trafficking
Article Snippet: .. Cells stably expressing the pSu9-Halo-mGFP mitophagy reporter, in addition to the indicated OPTN constructs, were further infected using lentiviral particles carrying YFP-Parkin–expressing plasmids (Addgene_23955) to ensure adequate mitophagy responses in HeLa cells. .. 24 h later these cells were then incubated with DMEM containing 10% FBS and 100 nm TMR HaloTag ligand (Promega) for 20 min at 37°C.

Homologous Recombination:

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

Non-Homologous End Joining:

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

Plasmid Preparation:

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer
Article Snippet: .. To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. Stable OVCAR4-sgNC, OVCAR4-CBX2 KO , OVCAR4-CBX2 IDRWT , OVCAR4-CBX2 IDRMut , CAOV3-sgNC, CAOV3-CBX2 KO , CAOV3-CBX2 IDRWT , CAOV3-CBX2 IDRMut cells containing the pLCN DSB Repair Reporter were generated through electroporation transfection (450Mv; 5 ms, Celetrix_LE).

Article Title: CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer.
Article Snippet: The primary antibody dilutions were anti-CBX2 (Cat#: MA5-38465, 1:200, ThermoFisher) and anti-EPCAM (Cat#: AB223582, 1:400, ABclonal). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477). .. HR and NHEJ assays To conduct integrated homologous recombination and non-homologous end-joining assays, the following plasmids were obtained from Addgene: pLCN DSB Repair Reporter (DRR) (Addgene plasmid #98895, RRID:Addgene_98895), pCAGGS DRR mCherry Donor EF1a BFP (Addgene plasmid #98896, RRID:Addgene_98896), and Isce1 (Addgene plasmid #26477, RRID:Addgene_26477).

Article Title: ASCL1 promotes nuclear shrinkage in transdifferentiation by suppressing NUP37
Article Snippet: .. We purchased the following plasmids from Addgene: pLKO.1/ p53 shRNA (#19119), pLKO.1/scrambled shRNA (#1864), pMD2.G (#12259), psPAX2 (#12260), pTight-9-124-Bclx (miR9/9 ∗ -124, #60857), pRL-SV40P (#27163), and pGL3 enhancer vector (#212938). .. The BAC clone containing the NUP37 genomic DNA was purchased from BACPAC resources (RP11-282A19).

Article Title: MeCP2 dysregulation inhibits mitophagy and impairs neural development in cortical organoids
Article Snippet: .. As previously described , equal proportions of mixed plasmids—pCXLE-hOCT3/4-shp53-F (Addgene #27077), pCXLE-hSK (Addgene #27078), and pCXLE-hUL (Addgene #27080)—were transfected into fibroblasts using Lipofectamine 3000 (Thermo Fisher, L3000015) at a ratio of 2 μg per plasmid. .. The medium was replaced with E8 medium (Cellapy, #CA1014500) the day after transfection, and the medium was changed every other day until iPSC clones emerged.

shRNA:

Article Title: ASCL1 promotes nuclear shrinkage in transdifferentiation by suppressing NUP37
Article Snippet: .. We purchased the following plasmids from Addgene: pLKO.1/ p53 shRNA (#19119), pLKO.1/scrambled shRNA (#1864), pMD2.G (#12259), psPAX2 (#12260), pTight-9-124-Bclx (miR9/9 ∗ -124, #60857), pRL-SV40P (#27163), and pGL3 enhancer vector (#212938). .. The BAC clone containing the NUP37 genomic DNA was purchased from BACPAC resources (RP11-282A19).

Transfection:

Article Title: MeCP2 dysregulation inhibits mitophagy and impairs neural development in cortical organoids
Article Snippet: .. As previously described , equal proportions of mixed plasmids—pCXLE-hOCT3/4-shp53-F (Addgene #27077), pCXLE-hSK (Addgene #27078), and pCXLE-hUL (Addgene #27080)—were transfected into fibroblasts using Lipofectamine 3000 (Thermo Fisher, L3000015) at a ratio of 2 μg per plasmid. .. The medium was replaced with E8 medium (Cellapy, #CA1014500) the day after transfection, and the medium was changed every other day until iPSC clones emerged.



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